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. 2018 Sep 10;19(9):2683. doi: 10.3390/ijms19092683

Figure 3.

Figure 3

Specific and high affinity binding of HM-3-Fc with integrin αvβ3 and α5β1. A flow cytometry method was setup with FITC-protein A (FITC-A) as a probe that binds the Fc part of HM-3-Fc. The dark line indicated the fluorescence signal for untreated synovial cells and it was used to define the threshold of positive fluorescence signal. The light line indicated the fluorescence signal for HM-3-Fc and FITC-A treated synovial cells. (A–C) The binding rates of synovial cells after incubation with 1, 10 or 75 μM HM-3-Fc before incubated with FITC-A. (D–F) The binding rates of synovial cells that were blocked with anti-αvβ3, anti-α5β1, or their combination.