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. 2018 Jul 4;8(3):64. doi: 10.3390/bios8030064

Figure 4.

Figure 4

Resistance and modelled parameters Rb, α, and Cm of brain endothelium grown in either Enriched Media or Minimal Media. Endothelial cells were seeded at 20,000 cells per well on a 96w20idf ECIS array. Time 0 h denotes the time cells were seeded. The dotted vertical line indicates 48 h of cell growth in each respective media type, with a subsequent media change carried out at this time. (A) Resistance at 4000 Hz trace over 120 h of cell growth; (B) Raw capacitance trace over 120 h of cell growth; (C) Modelled parameter, Rb, trace over 120 h of cell growth; (D) Modelled parameter, α, trace over 120 h of cell growth; (E) Modelled parameter, Cm, trace over 120 h of cell growth. Data show the mean ± SD (n = 3 wells) of one independent experiment representative of three experimental repeats. Graphical representations of p values are * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.