Figure 1.

Bland-Altman Plots resulting from the pairwise comparison of C_t values between triplex and monoplex direct real-time PCR assays by meningococcal serogroups: (a) NmA-csaB; (b) NmB-csb; (c) NmC-csc; (d) NmW-csw; (e) NmX-csxB; and (f) Nm-csy. Level of agreement between the two assays by serogroup (C_{t Triplex} − C_{t Monoplex}), also known as observed mean difference (small dash line), is presented as a function of the average C_t values of the two assays [(C_{t Triplex} + C_{t Monoplex})/2]. Reference lines (solid line) indicate the ideal zero difference. Upper and lower limits of agreement (large dash lines) defined by mean C_t difference +/− 1.96 × SD.