Table 1.
Characteristics of direct monoplex and triplex real-time PCR assays for meningococcal serogroup determination.
| Assay 1 | Fw/RV/Pb-Fluorophore 2 | QC Strains 3 | Monoplex LLD 4 (CFU/mL) | Triplex LLD (CFU/mL) | Triplex PP, PN 5 |
|---|---|---|---|---|---|
| AWX | 100% (75/75), 100% (45/45) | ||||
| NmA-csaB | 900/600/300 nM-HEX | M7060 | 5264 | 2221 | |
| NmW-csw | 300/100/200 nM-FAM | M7034 | 197 | 128 | |
| NmX-csxB | 600/600/200 nM-Quasar670 | M8210 | 178 | 68 | |
| BCY | 100% (108/108), 100% (45/45) | ||||
| NmB-csb | 900/300/200 nM-Quasar670 | M5178 | 420 | 286 | |
| NmC-csc | 300/300/300 nM-FAM | M3045 | 1780 | 1407 | |
| NmY-csxB | 900/300/300 nM-HEX | M2578 | 1232 | 765 |
1 Assay named for the included Neisseria meningitidis (Nm) serogroup, followed by capsule locus gene; 2 Fw, forward primer; RV, reverse primer; Pb, probe; 3 QC, quality strains used to determine LLD [5]; 4 LLD, lower limits of detection, defined as CFU/mL yielding a Ct value of 35; 5 Positive percent agreement (PP) and negative percent agreement (PN) calculated by using monoplex drt-PCR assays as a reference.