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. 2018 Jul 11;8(3):51. doi: 10.3390/biom8030051

Figure 2.

Figure 2

Live-cell confocal microscopy visualizes CPP-mediated β-lactamase internalization. The CPP-mediated uptake of CPP_SpyT/SpyC_BLA conjugates (4 µM) was visualized in CHO-K1 cells (a–f) and T47D cells (g–n). SpyC_BLA was conjugated to CPP_SpyT peptides. Unconjugated SpyC_BLA (No CPP negative controls) shows minimal evidence of internalization in both CHO-K1 and T47D cells (a,g,k). Conjugation with TAT_SpyT (b,h,l) or phylomer CPPs 1746c27_SpyT (c,i,m), 1746_SpyT (d,j,n), 0084_SpyT (e), and 0169_SpyT (f) enabled CPP-dependent uptake of SpyC_BLA. The degree of uptake is CPP-dependent and visualized by blue fluorescing cells. Bar scale is 50 µm (a–j) or 14.6 µm (k–n).