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. 2018 Sep 6;19(9):2638. doi: 10.3390/ijms19092638

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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BK phosphorylates FGFR-1 despite the absence of FGF-2. (A,B) HUVEC were treated with anti-FGF-2 neutralizing antibody (6 μg/mL), then stimulated with FGF-2 (20 ng/mL), as a positive control or BK (1 μM) for 10 min. FGFR-1 was immunoprecipitated (IP), and its activation was investigated by anti-pTYR antibody. Results were normalized to FGFR-1. *** p < 0.001 vs. Ctr (control, 0.1% FBS). (C) Murine EC was isolated from FGF-2^−/− mice were stimulated with BK (1 μM) for 10 min. FGFR-1 was immunoprecipitated (IP), and its activation was investigated by an anti-pTYR antibody. Results were normalized to FGFR-1. The results presented are representative of three independent experiments (n = 3) with similar results. *** p < 0.001 vs. Ctr (control, 0.1% FBS).