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. 2018 Sep 11;7(3):44. doi: 10.3390/biology7030044

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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P64 and its N-terminal (N-term) and C-terminal (C-term) domains condense Spodoptera frugiperda ascovirus-1a genomic (g)DNA. (A) SDS-PAGE showing purified 6x-histidine tagged proteins (rP64, rN-term, rC-term) used in the assays. (B) Examples of results of electrophoretic mobility shift assays (EMSA) showing the presence of SfAV-1a gDNA in supernatants (S) or pellets (P) after centrifugation following incubation without protein or with bovine serum albumin (BSA), rP64, rN-term and rC-term; ~2 μg of each protein and 15 ng of gDNA were used in each assay. Arrowhead indicates position of wells in agarose gels.