Figure 5.

TSPO expression in the hippocampal region of the normal mouse brain. (A) TSPO expression (red) and DAPI (blue) to label the distinctive structural features of the region. Strong TSPO immunoreactivity is observed within the subgranular zone of the dentate gyrus and the ependyma. (B) TSPO (red) colocalizes at low levels with Nestin+ cell bodies (green), but not processes, in the subgranular zone of the dentate gyrus. (C) TSPO expression (red) does not colocalize with GFAP+ astrocytes (green) of the hippocampus, though is present in GFAP+ neural stem/progenitor cells of the subgranular zone, further confirming the expression of TSPO in neural stem/progenitor cells. Scale bar = 300 µm for (A), 20 µm for (B,C).