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. 2018 Sep 13;11(9):1703. doi: 10.3390/ma11091703

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Figure A1 — Increasing the number of PEM bilayers: (a) percent LIVE^® stained area of MC3T3-E1s cultured on AntiA-bCaP-PEM30-FGF2 as compared to cells cultured on AntiA-bCaP-PEM102-FGF2 (* p < 0.05, ** p < 0.01 and *** p < 0.001); (b) fluorescent LIVE^® stained images at one, three, and five days of culture on AntiA-bCaP-PEM30-FGF2 as compared to cells on AntiA-bCaP-PEM102-FGF2; (c) percent LIVE^® stained area of MC3T3-E1s cultured on control bCaP-PEM30-FGF2 as compared to cells on bCaP-PEM102-FGF2 (*** p < 0.01); and (d) fluorescent LIVE^® stained images at one, three, and five days of culture on bCaP-PEM30-FGF2, as compared to cells cultured on bCaP-PEM102-FGF2. Scale bar = 100 μm.