Figure 4.

Treatment with SA4503 increases BDNF protein level in Atrx^ΔE2 mouse brain. (A) Representative immunoblot of mouse medial prefrontal cortex lysates probed with the indicated antibodies (top panel). Densitometry analysis of indicated proteins to β-tubulin (arbitrary units; (A.U.)) (bottom panel). * p < 0.05, versus vehicle-treated WT mice; ## p < 0.01, versus vehicle-treated Atrx^ΔE2 mice by one-way ANOVA with post hoc Tukey’s test; F (3, 16) = 13.7 in BDNF; n = 5 mice each. (B) Real-time RT-qPCR showing total BDNF mRNA (using exon IX primers) expression in mouse medial prefrontal cortex lysates. BDNF mRNA were determined relative to Gapdh mRNA. n = 5 mice for each group. The experiments were repeated two times with similar results. (C) Representative images of cultured cortical neurons at DIV5 with the mitochondrial-specific marker, MitoTracker Red. Images in the right panels are enlarged from the corresponding boxed areas. Scale bars: left panels, 50 µm; right panels, 3 µm. (D) Data show the mitochondrial length (left) and the roundness index (right); n = 20 cells each. Abbreviations: Veh., vehicle; SA, SA4503.