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Diagrams of the experimental schedule. In in vitro analysis using primary cortical neurons, SA4503 (1 µM, dissolved in distilled water) was treated for 48 h in cultured cortical neurons in both DIV5 and DIV24. In in vivo analysis using mice, we administered SA4503 over the course of two weeks (1 mg/kg, intraperitoneally (i.p.) daily from postnatal day (P) 70 to P84) to AtrxΔE2 mice and subsequently assessed memory-related behaviors and biological studies.
