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. 2018 Jul 13;6(3):64. doi: 10.3390/diseases6030064

Figure 4.

Figure 4

Budding of LFV-Z VLPs, eVP40 VLPs, and VSV-M40 virus from BAG3-WT or BAG3 knockout (KO) cells. (A) HAP1-BAG3-WT (WT) or HAP1-BAG3 knockout cells (KO) were transfected with LFV-Z, and the indicated proteins were detected in cell extracts and VLPs by Western blot analysis as shown in two independent experiments; (B) HAP1-BAG3-WT (WT) or HAP1-BAG3 knockout cells (KO) were transfected with eVP40, and the indicated proteins were detected in cell extracts and VLPs by Western blot analysis as shown in two independent experiments. The red arrows indicate the fold increase in budding of LFV-Z and eVP40 VLPs in BAG3-KO cells compared to BAG3-WT cells; (C) HAP1-BAG3-WT (WT) or HAP1-BAG3 knockout cells (KO) were infected with VSV-M40 recombinant virus, and the indicated proteins were detected in cell extracts by Western blot analysis as shown in three independent experiments. The bar graph depicts the average titers of infectious VSV-M40 virus from three independent experiments. VSV-M40 titers were log10 transformed before checking normality (via Shapiro Wilks normality test) and assessing equality of variance (via F-test). (** p = 0.003).