Table 1.
Primer pair sequences used to amplify Ceratitis capitata immune-related genes.
| Protein Product | Gene ID | Accession Number a | Primer Pair Sequences | Annealing Temperature (°C) |
|---|---|---|---|---|
| Heat shock protein | Hsp-70 | Y08955.1 | F: 5′CAAAGTGGAGATTATCGCCAACGACCA 3′ R: 5′CACTGTGATGACGGCGTCTGTTACTGT 3′ |
60 |
| SODCu/Zn | SOD1 | M76975.1 | F: 5′CCCGTCCTAGTCACTGGTGAGGTTAAC 3′ R: 5′CTTCGTTGCTCCATCGCCACTGG 3′ |
62 |
| Attacin-B | attacin | XM_004520300.2 | F: 5′TGGAGCTCGCTAAAGGTGTGGGCACA3′ R: ′CTTTGTGAGCGAATCACTTATGCCGGGTGT 3′ |
63 |
| Cecropin-1 | cecropin | XM_004534275.2 | F: 5′CTCCTCGCTGTCATCTTTGCCGTTTTC3′ R: 5′AGCAACATTGGCCGCCTGTTGGGC3 |
52 |
| Defensin-A | defensin | XM_004537571.2 | F: 5′TTATCGCCGGACTCATCCTCTGCTCCT3′ R: 5′ATGTACCGTTCCTGCAGTAACCGCCAC3′ |
63 |
| Integrin alpha-PS | integrin | XM_004521659.1 | F: 5′TGCAACCTGATCTGGGCGATTCGC3′ R: 5′ATGAGCTACTGCTATACGATGAGCCAGCCT3′ |
62 |
| Mucin C | mucin | XM_004533242.1 | F: 5′GGGGAGTACATAACCAATCCGGAAGCAACT3′ R: 5′GACTTGAGCGGTCGTTGTTGATGATGCAGT3′ |
60 |
| Ceratotoxin A | ceratotox | AJ272446.1 | F: 5′TAGTAGCCGAACCTGCTGCCGAAGATT3′ R: 5′AACGGGTAGAGCAGCCTTTGCAATGGGTAA3′ |
62 |
| NF-kB | NF-kB | XM_004517947.2 | F: 5′ACAAAGTTCTCAATGCCCACAATG3′ R: 5′GTTCCTTAACAGCGATATGTAGTGC3′ |
55 |
| SCP_17a | SCP_17a | DQ406807.1 | F: 5′AATATTCTTGTCGGCAACCATAA3′ R: 5′CAGAATTTGGCATGGATCAGT3′ |
50 |
| JHBP_28C | JHBP_28C | DQ406809.1 | F: 5′TGAACGGACCAGATATCCAA3′ R: 5′ACCATGCAGATAGGCAGGAC3′ |
52 |
a GenBank accession number of sequences used for primer design.