Table 1.
Primary antibodies | ||||
Antigen | Code | Species | Working dilution | Supplier |
CGRP | AB5920 | Rabbit | 1:1600 | Chemicon Int Temecula, OH, USA |
GAL | T-5036 | Guinea Pig | 1:2000 | Peninsula San Carlos, CA, USA |
SP | 8450-0505 | Rat | 1:1000 | Bio-Rad (AbD Serotec), Kidlington, UK |
Secondary antibodies | ||||
Reagents | Working dilution | Supplier | ||
Alexa fluor 546 donkey anti-rabbit IgG | 1:1000 | Invitrogen Carlsbad, CA, USA | ||
Alexa fluor 546 donkey anti-guinea pig IgG | 1:1000 | Invitrogen | ||
Alexa fluor 488 goat anti-rat IgG | 1:1000 | Invitrogen |