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. 2018 Sep 14;19(9):2765. doi: 10.3390/ijms19092765

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Elutriation of K562 cells separates the cells according to size. (A) FACS analysis (Flowjo) of each elutriation fraction showing the percentage of G1, S or G2/M phase cells. (B) Immunofluorescence analysis of each elutriation fraction showing the percentage of each fraction in lateS/G2/M determined by CENPF immunofluorescence and the percentage of mitotic cells determined by DAPI staining. (C,D) TOP2A and TOP2B protein level per cell was determined by quantitative immunofluorescence [77], the data shown represent the mean ± SEM of median values obtained from three replicate experiments. Statistical analysis was carried out using one way ANOVA (* p < 0.05; *** p < 0.0001).