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. 2018 Sep 7;10(9):478. doi: 10.3390/v10090478

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Light microscopic findings in the cerebrum of white-tailed sea eagles. (A) Mild, acute, oligo- or multifocal, necrotizing polioencephalitis characterized by condensed, hypereosinophilic neuroglial cells with pyknotic and karyorrhectic nuclei (arrows) represents a typical lesion. Haematoxylin–eosin; Bar = 50 µm. (B) Immunohistochemistry confirmed the presence of influenza A virus nucleoprotein antigen within neuroglial nuclei (arrows) within the lesions. Polyclonal rabbit anti-influenza A FPV/Rostock/34-virus-nucleoprotein antiserum; avidin-biotin-complex method; 3-amino-9-ethyl-carbazol chromogen (red-brown); haematoxylin counterstain (blue); Bar = 50 µm.