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. 2018 Jun 26;5(3):63. doi: 10.3390/vetsci5030063

Figure 5.

Figure 5

Results of DNA tests for all batches of canine and feline Anallergenic™ diets intended for commercial use (subject to being below the contamination threshold) from 2011 to April 2018. These did not include samples from equipment rinsing batches or batches from the preliminary steps of industrialization of production. More than 99.9% of the tested batches conformed to the contamination limits, having ancillary protein-related DNA content either not detectable (n = 1560, 72.3%), below the total DNA conformity threshold of 1.2 µg/g (n = 555, 25.7%), or above the total DNA conformity threshold but below a source-specific NPPI of 0.5% based on qRT-PCR and DNA-protein calibration curves (n = 42, 1.95%). In total, less than 0.1% of the tested batches (n = 2/2159) did not conform to the standards and were then discarded. LOD, limit of detection = 0.003 µg/g; NPPI, no protein pollution index; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction. * Over the threshold specification used for maximum acceptable levels of ancillary protein, hence products were discarded.