Figure 3.

Effects of D14 and D15 on the proteolytic activity of matrix metalloproteinases (MMP)-2 and MMP-9 in osteosarcoma cells. U2OS and SAOS-2 cells were treated with D14 and D15 in serum-free medium at the indicated concentrations for 24 h. Next, the supernatant (conditioned medium) was collected and subjected to gelatin zymography to analyze the activity of secreted MMP-2 (A) and MMP-9 (B). Quantitative results of three independent experiments are expressed as means ± standard deviation. Means followed by the same letter are not significantly different (p < 0.05) according to Tukey’s HSD test.