Table 1.

Relative expression levels of novI and novR by quantitative reverse transcription polymerase chain reaction (qRT-PCR). RNA samples were extracted from bacterial cultures after 24 h, 36 h, 48 h and 72 h. The levels of gene expression in US6-1, ∆rsh, ∆rsh (pCM62-rsh) were normalized to 16S rRNA gene, and the relative levels in ∆rsh and ∆rsh (pCM62-rsh) to that in the wild-type strain (set as value of 1) were reported. Values shown are the average of biological triplicate experiments with standard deviations (mean ± standard deviation (SD)). “*” represents p < 0.05; “**” represents p < 0.01; “***” represents p < 0.001.

Genes	Strains	24 h	36 h	48 h	96 h
novI	US6-1	1.166 ± 0.773	1.060 ± 0.466	1.115 ± 0.647	1.039 ± 0.356
	∆rsh	0.261 ± 0.083	0.011 ± 0.002 *	0.020 ± 0.004 *	0.026 ± 0.004 **
	∆rsh (pCM62-rsh)	0.501 ± 0.085 ***	0.648 ± 0.216	0.753 ± 0.097	3.975 ± 1.547 *
novR	US6-1	1.012 ± 0.185	1.006 ± 0.129	1.001 ± 0.055	1.029 ± 0.314
	∆rsh	0.119 ± 0.018 **	0.068 ± 0.005 ***	0.547 ± 0.017 ***	0.881 ± 0.354
	∆rsh (pCM62-rsh)	0.601 ± 0.050 **	0.463 ± 0.076	0.543 ± 0.054	2.486 ± 0.972