Table 2.
Bacterial strains and plasmids used in this study.
| Strains or Plasmids | Relevant Traits | Source or Reference |
|---|---|---|
| Strains | ||
| N. pentaromativorans | ||
| US6-1 | Wild type (JCM 12182) | Microbe division JCM |
| ∆rsh | US6-1, rshUS6-1 deletion mutant, Rifr | This study |
| ∆rsh (pCM62-rsh) | ∆rsh with the plasmid pCM62-rsh, Tcr | This study |
| ∆novI | US6-1, novI deletion mutant, Rifr | Lab stock |
| E. coli | ||
| DH5α | F- hsdR17 endA1 thi-1 gyrA96 relA1 supE44∆lacU169 (ψ80dlacZ∆M15) | TransGen Biotech |
| S17-1 (λpir) | E. coli K-12 Tpr Smr recA thi hsdRM+ RP4::2-Tc::Mu::Km Tn7, λpir phage lysogen | [62] |
| A. tumefaciens A136 (pCF218) (pCF372) | traI-lacZ fusion; AHL biosensor; Tcr Spr | [63] |
| Plasmids | ||
| pMD19-T | T-vector, Ampr | TaKaRa, Dalian, China |
| pAK405 | Plasmid for allelic exchange and markerless gene deletions; Kmr | [64] |
| pAK405-rsh | pAK405 with fusion of up- and downstream regions of rshUS6-1; Kmr | This study |
| pCM62 | Broad-host-range cloning vector; IncP origin of replication; Tcr | [65] |
| pCM62-rsh | pCM62 with rshUS6-1 ORF and 502 bp of upstream region; Tcr | This study |