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. 2018 Aug 28;19(9):2549. doi: 10.3390/ijms19092549

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Adipose-derived stromal cell (ASC) morphology and proliferation. Exemplary phase contrast images of ASC monolayer cultures in different inflammatory environments ((a) control; (b) IL-1β-high (10 ng/mL); (c) IL-1β-low (0.01 ng/mL); (d) TNF-α-high (50 ng/mL); (e) TNF-α-low (0.1 ng/mL); (f) IL-1β-high + TNF-α-high; (g) IL-1β-low + TNF-α-low; (h) co-culture with activated leukocytes (Leuko-stim); (i) co-culture with non-activated leukocytes (Leuko)) obtained at Day 1, and boxplot displaying confluency as a measurement of ASC proliferation. Data were obtained by quantitative analysis of phase contrast images from Day 1 and Day 3. Groups displayed by boxes sharing the same letter are significantly different from each other (p < 0.05; n = 7).