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. 2018 Sep 3;10(9):472. doi: 10.3390/v10090472

Figure 2.

Figure 2

Effects of MMDaV-encoded proteins on potato virus X (PVX) pathogenicity. (A) Symptoms of Nicotiana benthamiana plants inoculated with Agrobacterium cells harboring PVX alone, or recombinant PVX vector expressing individual ORFs of MMDaV. Photographs were taken at 10 days post infiltration (dpi). Experiments were repeated three times, and at least four to six plants were used for each inoculation. (B) Western blot analysis of PVX accumulation in inoculated N. benthamiana plants at 7 to 10 dpi using a monoclonal antibody against PVX CP. Total proteins were extracted from upper non-inoculated leaves as indicated in (A). Two independent plants were used to extract total proteins. H represents total soluble proteins extracted from the healthy N. benthamiana plant, which was used to detect the specificity of the PVX antibody. Ponceau staining of the large subunit of Rubisco served as loading controls.