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Imaging of retina in control animal experiments where rats received only phosphate buffered saline intravitreally. As expected, no fluorescence can be detected either in vivo (ICON) (a) or in the ex vivo retina whole mount preparation (b) (red channel for rhodamine 123 detection). (c) The retina whole mount, nuclei staining by Hoechst 33342. Scale bars: (a) 200 µm; (b,c) 50 µm.
