Skip to main content
. 2018 Sep 5;19(9):2632. doi: 10.3390/ijms19092632

Figure 1.

Figure 1

Effect of the long-term administration of a grape seed proanthocyanidin extract (GSPE) with an obesogenic diet on the size and number of visceral adipocytes. Rats were fed either a standard chow diet (STD group, n = 10), a cafeteria diet (CAF group, n = 10), or a cafeteria diet with daily administration of 25 mg GSPE/kg BW, 100 mg GSPE/kg BW or 200 mg GSPE/kg BW (GSPE 25, GSPE 100, and GSPE 200, n = 10 per group) for 12 weeks. (a) Representative light microscopy images of retroperitoneal white adipose tissue (rWAT) stained with hematoxylin and eosin from each group; (b) Adipocyte area measured from the representative light microscopy images from each group; (c) Adipocyte volume calculated from the adipocyte area; (d) Total adipocyte number extrapolated from the size of adipocytes and the weight of rWAT; (e) Frequency of adipocyte size calculated from the adipocyte area. The values are the means ± SEM of five fields per animal from three animals of each group. Different letters indicate significant differences between groups using one-way ANOVA. Significance was considered when p ≤ 0.05.