Table 3.

Flow cytometric analysis of immune and epithelial cell proteins in HM cells (%) taken from healthy, mastitis and non-mammary post-surgery participants. Double stainings between either CD45 or EPCAM positive cells and immune proteins.

	Healthy (n = 12)	Mastitis Sample (n = 1)		Non-Mammary Surgery Sample (n = 1)
	Median (Range)	Mastitis Breast	Adjacent Breast
CD45–Granzyme A (%)
All single cells	0.2 (0.0–14.9)		0.2	0.4
Population 1	1.0 (0.3–32.6)		10.9	5.4
Population 2	0.2 (0.0–50.0)		3.5	1.3
Population 3	0.2 (0.0–0.4)		0.2	0
CD45–Granzyme B (%)
All single cells	0.7 (0.0–5.5)	0.5	0.4	4.4
Population 1	5.2 (0.0–26.8)	27	17.2	33.4
Population 2	0.3 (0.0–13.9)	0.3	4.2	20.3
Population 3	0.0 (0.0–0.3)	0	0.3	1.4
CD45–Granulysin (%)
All single cells	0.7 (0.0–10.4)		0.3	4.1
Population 1	3.2 (0.8–21.1)		4.4	12.9
Population 2	3.6 (0.0–13.4)		5.8	34
Population 3	13.6 (0.2–27.1)		0.1	0.9
CD45–Perforin (%)
All single cells	0.9 (0.6–8.3)	0.8	0.9	23.7
Population 1	11.0 (0.1–35.2)	34.1	8.2	27.7
Population 2	2.1 (0.1–26.8)	3.9	10.6	48.2
Population 3	0.7 (0.1–1.8)	0.7	0.1	4.8
EPCAM–Granzyme A (%)
All single cells	0.0 (0.0–0.2)		0.1	0
Population 1	0.1 (0.0–35.2)		0.1	0.1
Population 2	0.4 (0.0–1.5)		0.6	0
Population 3	1.0 (0.2–1.8)		0.2	0
PCAM–Granzyme B (%)
All single cells	0.2 (0.1–1.0)	1.3	0	1.5
Population 1	0.5 (0.3–3.5)	2.2	0.6	0.9
Population 2	1.4 (0.0–3.0)	0.3	0.5	6.1
Population 3	1.3 (0.2–2.3)	0	0.2	1.1
EPCAM–Granulysin (%)
All single cells	0.2 (0.0–2.1)		0.1	2.4
Population 1	1.5 (0.2–3.1)		1.8	1
Population 2	0.6 (0.1–1.4)		1.4	7.9
Population 3	2.6 (0.2–5.0)		0.1	1.9
EPCAM–Perforin (%)
All single cells	0.2 (0.0–5.5)	0.2	0.1	5.2
Population 1	3.3 (1.1–9.5)	2.1	0.5	2.2
Population 2	1.7 (0.3–8.4)	0.7	1.1	7.4
Population 3	0.4 (0.3–0.4)	0.3	0	1.8