Figure 1.

Antiproliferative action of metformin in SKOV-3 cells is reduced by hyperglycemia. SKOV-3 cells were cultured in normoglycemia (A) and hyperglycemia (B) and simultaneously treated with 10 mM metformin for 24, 48, 72 h. The cell number was determined using the trypan blue method and the proliferation rates were calculated (C). NG + M—cells cultured in normoglycemia and treated with metformin, HG + M—cells cultured in hyperglycemia and treated with metformin, td—proliferation rate. Results are presented as means ± SD of four independent experiments. (*) Statistically significant differences in comparison to untreated cells at time point “0”, p < 0.05; (#) statistically significant differences between the cells exposed to metformin in comparison to unexposed cells at the same time point, p < 0.05.