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Tartrate-resistant acid phosphatase (Trap) stain of the osteoclast. Calcitriol inhibits the fusion ability and the number of TRAP-positive differentiated osteoclast cell in a dose-dependent manner. Multinuclear TRAP-positive cells containing more than three nuclei were scored as osteoclasts. Data are mean number ± SD of osteoclasts. (* p < 0.05 versus control group, n = 3).
