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Wnt10b and Wnt 16 protein expression in calcitriol-treated osteoblasts. After preosteoblast 7F2 cells were stimulated with 100 μg/mL ascorbic acid and 10 mM β-glycerol phosphate, Western blot analysis showed both Wnt 10b and Wnt 16 expression in osteoblast at different doses of calcitriol was not significantly different at the protein level. Actin protein served as a loading control. Data are means ± SD (n = 3).
