Figure 2.

Validation of mechanical and cellular properties. (A) Sample waveform depicting the oscillatory pressure waves observed within the center of the channel. (B) WT and mutant Bmpr2^R899X endothelial cell channels imaged at 5× with fluorescent calcein AM to observe the monolayer formed by the cells. Channels were imaged after perfusion to ensure that the endothelial monolayer remained intact after the flow experiments. Cells were removed from conditional immortalization and the transgene was activated though doxycycline.