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. 2018 Sep 12;16(9):332. doi: 10.3390/md16090332

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Distribution of the clones from the archaeal 16S rRNA clone library among different genera. 16S rRNA fragments obtained by amplification with archaeal specific primers were cloned into pGEMT vector for the construction of a clone library. The inserted sequence of 25 of the obtained clones were analysed and compared with the NCBI to identify the original genera. Data are expressed as percentages of the total archaeal population. Only sequences that shared over 95% 16S rRNA sequence identity with a known one was assigned to a specific genus.