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. 2018 Sep 10;19(9):2694. doi: 10.3390/ijms19092694

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Gene transduction efficacy of adenovirus (Ad)5/35 is enhanced in CD46-expressing cells. (A) Western blot analysis of CAR and CD46 expression in parental rodent baby hamster kidney (BHK) cells or BHK-CAR and BHK-CD46 cells, which ectopically express the Ad receptors CAR and CD46, respectively. (B) Flow cytometry analysis of Ad-mediated green fluorescent protein (GFP) expression in BHK, BHK-CAR, and BHK-CD46 cells. Transduction of GFP by Ad5 (left panel) or Ad5/35 (right panel) analysis was measured by flow cytometry. (C) A cell killing assay was performed for all three BHK cell lines using either Ad5-tk (left panel) or Ad5/35-tk (right panel) followed by ganciclovir (GCV) treatment. Cytotoxicity was analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Error bars represent standard error (SEM). Statistics: C, p < 0.01 by two-way analysis of variance (ANOVA). Confirmed.