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. 2018 Sep 10;19(9):2694. doi: 10.3390/ijms19092694

Figure 4.

Figure 4

CD46 expression analysis and Ad5/35-mediated gene transduction in bladder cancer cell lines. (A) CAR and CD46 expression levels were analyzed in bladder cancer cell lines by Western blot analysis. (B) Transduction analyses of bladder cancer cell lines by flow cytometry following infection with different doses of Ad5-GFP or Ad5/35-GFP. (C) Transduction analyses of bladder cancer cell lines by fluorescence microscope following infection with the indicated multiplicities of infection (MOI) of Ad5-GFP or Ad5/35-GFP. At 24 h post-infection, GFP expression levels were monitored (20×).