Figure 7.

CD46 promotes Ad5/35-tk-mediated cytotoxicity of xenograft bladder tumor growth in vivo. (A) CD46 overexpressing (CD46), CD46 suppressed (shCD46), or control EJ bladder cancer cells (veh) were injected subcutaneously into nude mice. Intra-tumoral injections of Ad5/35-tk viruses (1.5 × 10⁸ plaque-forming units (PFU)) were performed twice at the indicated time points followed by intraperitoneal GCV injections (75 mg/kg) on days 2–12. Tumor growth was measured by a caliper at the indicated time points. (B) Representative photograph of tumor xenografts from mice treated with either Ad5-tk/GCV or Ad5/35-tk/GCV as described in (A). (C) Tumor tissue samples from the different tumor types and treatments were evaluated for CD46 expression by Western blot analysis. (D) Cell proliferation of each of different EJ cells was measured using the MTT assay. The error bars represent SEM. Statistics: A, p < 0.05 by two-way ANOVA.