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. Author manuscript; available in PMC: 2018 Sep 30.
Published in final edited form as: Int J Biochem Cell Biol. 2010 Aug 5;42(11):1802–1807. doi: 10.1016/j.biocel.2010.07.018

Fig. 3.

Fig. 3

Dorsomorphin (DM) and LDN-193189 (LDN) inhibit BMP2 mediated activation of Smad and non-Smad signalling cascades. Serum starved C2C12 cells were treated with 5 µM DM (A) and 0.5 µM LDN (B) for 30 min prior to addition of 5 nM BMP2. Final DMSO concentration was adjusted to 0.05% in DM treated and 0.005% in LDN treated samples. Cells were lysed at indicated timepoints and subsequently probed for western blotting using different antibodies against p-Smad1/5/8 and the non-Smad signalling targets p-p38, p-Akt, p-ERK1/2, p-ATF2 and p-CREB. Each membrane was also probed against tubulin or GAPDH as loading control.