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. 2018 Aug 30;10(9):466. doi: 10.3390/v10090466

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Time course of BKPyV pre-miRNA expression in infected RPTECs. (A) Schematic representation of the BKPyV pre-miRNA hairpin and the corresponding BKPyV-miRNA-5p and miRNA-3p sequence (underlined). (B) Time course of BKPyV pre-miRNA expression levels after infection of RPTECs with the indicated strains. Mean values ± standard deviation of 3 independent experiments are shown. (C) Comparison of relative BKPyV pre-miRNA expression levels per BKPyV genome copy (template) in cells. Results are expressed as BKPyV-miRNA levels normalized to BKPyV genome copy number, archetype WW(1.4) values at 12 h post-infection are set to 1 (-fold; ** p < 0.01).