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. 2018 Sep 6;19(9):2652. doi: 10.3390/ijms19092652

Figure 3.

Figure 3

Plant polyphenols suppress anti-EGFR drug-induced IFN-κ and IFIT1 expression in human keratinocytes. (A,B) quantitative real-time RT-PCR and (C,D) Western blot detection in whole-cell extracts of IFN-κ and IFIT1. Human keratinocytes were pretreated with resveratrol (Resv) or quercetin (Querc), both at 50 µM concentration, for 30 min and then stimulated with cetuximab (Cetux, 25 μg/mL) or gefitinib (Gefit, 2 µM) for further 6 h, followed by RNA or protein extraction. In (A,B), data are expressed as the mean ± S.D. (standard deviation) (n = 15 per condition) of transcript fold induction compared to untreated controls (−). * p < 0.05 versus untreated controls; # p < 0.01 versus Cetux-treated conditions; § p < 0.01, versus Gefit-treated conditions. (C) Western blot detection in whole-cell extracts of IFN-κ and IFIT1. (D) The ratios of IFN-κ and IFIT1 protein levels to their loading control (actin) were calculated. * p < 0.05, vs. untreated controls; # p < 0.05 versus Cetux; § p < 0.05, vs. Gefit. (A,B,D), data are from three independent experiments, and are indicative of experiments on cells from four different healthy donors.