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Oxidative folding of native and diselenide analogs of Hv1a. (A–D): RP-high-performance liquid chromatography (HPLC) chromatograms showing the native and each of the diselenide analogs of Hv1a after oxidative folding. The folding conditions were: 0.2 M MOPS buffer, pH 7.3, 0.4 M KCl, 2 mM EDTA, 10 mM GSH/2 mM GSSG, 24 h, room temperature. Asterisks denote the peak corresponding to the correctly folded diselenide toxin.
