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Selenite reduction assay on different subcellular fractions (cytoplasmic, periplasmic, and membrane), supernatant, and exopolysaccharide. All experiments were performed in duplicate (indicated by roman numbers), with addition of 5.0 mM SeO32− and 2.0 mM NADH or NADPH. While 3 following control negatives were performed: without protein fractions or supernatant or EPS, without selenite, without NADH or NADPH.
