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. 2018 Sep 14;19(9):2770. doi: 10.3390/ijms19092770

Figure 5.

Figure 5

The effect of FFO extract on the level of Wnt/β-catenin signaling proteins. (A) DPCs were stimulated with minoxidil (10 μM) or FFO extract (25 μg/mL) for 18 h. The levels of phospho-GSK3β and GSK3β were determined using an immunoblot. The graph represents the quantitative level of the proteins. The data are presented as the mean ± SD from three independent experiments. * p < 0.05 vs. vehicle-treated control. (B) DPCs were stimulated with minoxidil (10 μM) or FFO extract (25 μg/mL) for 56 h. Cytoplasmic and nuclear fractions were prepared as described in Section 4. The protein level of β-catenin was determined using an immunoblot. α-Tubulin and lamin B1 were used to confirm the same loading of cytoplasmic and nuclear proteins. The graph represents the quantitative level of the proteins.