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Effect of DMY on sirtuin 3 (SIRT3) expression in the myocardium of mice after TAC. (A) SIRT3 mRNA expression was quantified by real-time PCR. 18S was used as a housekeeping gene; (B) SIRT3 protein expression was quantified by western blot. β-tubulin was used as a loading control; (C) SIRT3 activity was quantified with fluorimetry. ** p < 0.01 versus Sham; # p < 0.05, ## p < 0.01 versus TAC (n = 5–8).
