Figure 3.

CDK1 is responsible for LMN A/C phosphorylation in mouse oocyte. (a) Western blot analysis of oocyte samples treated for 2 h after NEBD with 20 µM CDK1 inhibitor, Roscovitine (Rosco). Phosphorylation status of LMN A/C (Ser22) was detected using a specific antibody. Antibodies against LMN A/C and GAPDH were used as a loading control. (b) Quantification of total and phosphorylated LMN A/C after Roscovitine (Rosco) treatment. Protein levels were normalized in a way that non-treated controls are 100%. Data was derived from three experiments containing biologically different samples. Columns represent mean, ± SD; ns non-significant; * p < 0.05, Student’s t-test.