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. 2018 Sep 19;19(9):2841. doi: 10.3390/ijms19092841

Figure 5.

Figure 5

Translation of a number of translational factor components is increased in the oocytes from aged females. (a) mRNA abundance of MPF components, Cdk1 and Cyclins B as well as Gapdh used as the control in the polyribosomal fractions. Percentage of reads from RNA-Seq. See Figure S3a for quantification of total RNA in oocytes from YF and AF groups. Three independent experimental datasets from biologically different samples. Values obtained for the YF group were set as 100%. Data columns represent mean; error bars, ± SEM; ns non-significant; *** p < 0.001; Student’s t-test. (b) Top 10 most enriched GO Function categories in polysome-bound mRNAs in YF (grey) and AF (red) oocytes determined by Gorilla. NoG (Number of Genes) denotes the number of genes in enriched categories. Highlighted lines represent translational functional categories. (c) Translational regulation of mRNA coding for different translational factors from polysomal fractions of YF and AF oocytes. Values obtained for the YF group were set as 100%. Data represent mean ± SEM; * p < 0.05; ** p < 0.01; *** p < 0.001; Student’s t-test.