Figure 4.

MHY2256 significantly reduces the growth of Ishikawa endometrial cancer cells in nude mice. Mice with pre-established tumors were randomized into three groups, with five mice in each group. The vehicle control, MHY2256 (5 mg/kg, twice/week, intrperitoneal (i.p.) injection), and salermide (30 mg/kg/week, i.p.) were administered to the tumor-bearing mice. (A) The mean tumor volumes during the 30-day treatment for each treatment group. (B) Each bar represents the inhibition rate (% of control) of the mean tumor weight. (C) The tumors were fixed in 10% formalin and embedded in paraffin. Immunohistochemical staining for Ki-67 was measured in the tumors. Magnification = ×200. Scale bar = 50 μM. The representative images were recorded under a 40× objective lens. In all graphs, the results are presented as the mean ± SE per group. Statistical analysis was performed using one-way analysis of the variance (ANOVA), followed by Bonferroni’s multiple comparison test. * p < 0.05 and ** p < 0.01 indicate significant differences between the control and treatment groups.