Table 2.

Relative lipid contents of individual classes in percent.

Lipid Class %	Group 1 Normal TAG, wt PNPLA3	Group 2 Normal TAG, var PNPLA3	Group 3 High TAG, wt PNPLA3	Group 4 High TAG, var PNPLA3
TAG	25.63 ± 12.49	35.22 ± 15.30	68.64 ± 11.26 ***	69.82 ± 11.86 ***
DAG	0.60 ± 0.30	0.57 ± 0.17	0.92 ± 0.12 *	1.02 ± 0.20 **
FFA	7.23 ± 3.57	6.07 ± 1.71	2.60 ± 1.12 ***	2.37 ± 1.01 **
CER	0.26 ± 0.04	0.25 ± 0.08	0.10 ± 0.03 ***	0.10 ± 0.05 ***
DCER	0.03 ± 0.01	0.03 ± 0.01	0.01 ± 0.00 ***	0.01 ± 0.01 ***
HCER	0.07 ± 0.02	0.05 ± 0.02	0.03 ± 0.01 ***	0.02 ± 0.01 **
LCER	0.06 ± 0.01	0.06 ± 0.03	0.04 ± 0.02	0.03 ± 0.01
PC	38.51 ± 9.74	33.06 ± 8.63	14.86 ± 6.41 ***	14.75 ± 7.83 ***
LPC	0.66 ± 0.22	0.60 ± 0.14	0.24 ± 0.14 ***	0.28 ± 0.17 **
PE	20.43 ± 5.80	17.84 ± 5.82	8.19 ± 3.24 ***	7.29 ± 3.10 ***
LPE	0.16 ± 0.05	0.15 ± 0.03	0.06 ± 0.03 ***	0.07 ± 0.04 ***
CE	2.45 ± 0.34	2.84 ± 0.45	2.73 ± 0.60	2.86 ± 0.48
SM	3.91 ± 0.81	3.28 ± 0.81	1.59 ± 0.67 ***	1.37 ± 0.60 ***

TAG: triacylglycerols, DAG: diacylglycerols, FFA: free fatty acids, CER: ceramides, DCER: dihydroceramides, HCER: hexosylceramides, LCER: lactosylceramides, PC: phosphatidylcholines, LPC: lysophosphatidylcholines, PE: phosphatidylethanolamines, LPE: lysophosphatidylethanolamines, CE: cholesterol esters, SM: sphingomyelins; wt: wild-type allele with I148I; var: I148M variants, which encode I148M (heterozygous) or M148M (homozygous) variants, respectively. Numbers denote averages ± standard deviations. * denotes significant differences between group 3 vs. 1 or group 4 vs. 2 illustrating the effect of liver TAG content; * p < 0.05, ** p < 0.01, *** p < 0.001. Significance was calculated using ANOVA with Holm-Sidak´s post-hoc test and assumed as p < 0.05. By the comparisons of group 2 vs. 1 and group 4 vs. 3 no significant differences were found for I148M PNPLA3 variant vs. wild-type carriers.