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. 2018 Aug 23;19(9):2485. doi: 10.3390/ijms19092485

Figure 2.

Figure 2

Vismodegib decreases hedgehog (Hh) target gene glioma-associated oncogene homologue 1 (GLI1) and Survivin expression. (A) Time schedule of vismodegib application and RNA/protein extraction for analysis. BCC-1 or SCC-25 cells were plated 24 h before treatment with 10 or 40 µM vismodegib or with DMSO as control for 24 h or 48 h before analysis. (B) mRNA expression for GLI1 and Survivin (C) relative to DMSO-treated controls. n = 2 (in duplicate); * p < 0.05, ** p < 0.01 (vismodegib- versus DMSO-treated cells, t-test). (D) Representative Western blots from at least two independent experiments, including detection of p53 expression in the cell lines. β-actin served as loading control. Rel., relative; Vism., vismodegib.