Table 1.
Radiation response variables of vismodegib-treated BCC-1 and SCC-25 cells.
| Cell Line Treatment | Plating Efficiency [%] | α [Gy−1] | β [Gy−2] | Radiation Dose at 50% Cell Survival [Gy] | Sensitizer Enhancement Ratio (versus DMSO Control) | Radiation Dose at 10% Cell Survival [Gy] | Sensitizer Enhancement Ratio (versus DMSO Control) |
|---|---|---|---|---|---|---|---|
| BCC-1 | |||||||
| DMSO | 23.29 ± 3.39 | 0.0428 | 0.0307 | 4.10 | 7.99 | ||
| 5 µM Vism. | 25.00 ± 3.67 | 0.2348 | 0.0074 | 2.72 | 1.51 | 7.85 | 1.02 |
| 10 µM Vism. | 24.29 ± 4.84 | 0.2044 | 0.0147 | 2.82 | 1.45 | 7.37 | 1.08 |
| 40 µM Vism. | 21.11 ± 1.54 | 0.2724 | 0.0084 | 2.37 | 1.73 | 6.96 | 1.15 |
| SCC-25 | |||||||
| DMSO | 28.56 ± 2.52 | 0.0849 | 0.0290 | 3.64 | 7.57 | ||
| 5 µM Vism. | 29.44 ± 1.67 | 0.2479 | 0.0138 | 2.46 | 1.48 | 6.75 | 1.12 |
| 10 µM Vism. | 31.53 ± 5.59 | 0.3189 | 0.0104 | 2.04 | 1.79 | 6.03 | 1.25 |
| 40 µM Vism. | 22.39 ± 2.43 | 0.3152 | 0.0143 | 2.02 | 1.81 | 5.79 | 1.31 |
The linear quadratic equation (SF = exp [−α × D − β × D2]) with fitted α and β values of the individual survival curves was transformed to calculate radiation-induced cytotoxicity enhancement factors at 50% and 10% cell survival versus DMSO controls. D, radiation dose [Gy]; DMSO, control cells treated with equivalent amounts of DMSO; SF, surviving fraction; Vism., vismodegib-treated cells using indicated concentrations.