Figure 5.

Expression patterns of individual XTH genes in response to flooding and ACC treatment studied by qRT-PCR or RNA-seq. (A) Expression pattern of individual XTH genes in response to 24 h flooding treatment in root and hypocotyl organs of two-day-old soybean seedlings. (B,C) Organ-specific expression analysis showed that most XTH genes were unregulated by the ACC in the root tissue, but there was no significant difference in the aerial parts of the three-week-old soybean. Three flooding-related homologous marker genes in soybean were studied as positive controls. Fold change (Log2) of relative gene expression (Actin (Glyma.18G290800)) of soybean was used as the normalization control.