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. 2018 Sep 11;10(9):488. doi: 10.3390/v10090488

Figure 5.

Figure 5

Subcellular localization of AGV C4 mutant variants on N-terminal acylated sites in N. benthamiana leaves. The recombinant fluorescent protein C4G2A-GFP expressed from pCam35S-C4G2A-GFP is mainly localized to chloroplasts, C4C8A-GFP expressed from pCam35S-C4C8A-GFP is targeted to plasma membrane, and nucleus and C4G2AC8A-GFP expressed from pCam35S-C4G2AC8A-GFP is localized in the cytoplasm, nucleus, and chloroplasts. The chloroplasts are identified by the red auto-fluorescence of chlorophyll. Confocal laser scanning images were taken approximately 3 d post infiltration. Scale bars indicated in the photos represent 20 μm.