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. 2018 Sep 11;10(9):488. doi: 10.3390/v10090488

Figure 6.

Figure 6

The N-terminal acylated residues of C4 are associated with its symptom determinant function. (a) Symptoms of N. benthamiana plants inoculated with PVX, PVX-C4, and PVX-C4 mutant variants mutated in its N-terminal potentially acylated sites. Symptoms were photographed at 21 dpi. The images shown are representative plants out of six plants inoculated with each construct in at least three independent experiments. PVX-C4G2A showed PVX-C4-like phenotype with severe upward leaf curling, PVX-C4C8A induced very mild upward leaf curling, and PVX-C4G2AC8A-infected plants showed PVX-like symptoms. (b) Northern blotting analyses of viral RNA accumulation showed that there were no obvious differences among PVX-C4 mutant variants-inoculated plants compared to PVX- and PVX-C4-inoculated plants. The blot shown is a representative one out of three independent experiments. The probe used was specific for PVX RNA.