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. 2018 Sep 18;19(9):2825. doi: 10.3390/ijms19092825

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Anti-melanogenic effect of loliolide. (a) Measurement of cell viability by MTT assay in loliolide-treated B16F10 cells. (b) Measurement of melanin secretion by melanin secretion assay in B16F10 cells treated with α-MSH and loliolide. (c) Measurement of melanin production by melanin content assay in B16F10 cells after treatment with α-MSH and loliolide. (d) Western blot analysis of the expression of MC1R signaling pathway proteins in α-MSH- and/or loliolide-treated B16F10 cells. Statistical significance (b,c) was evaluated using the Kruskal–Wallis/Mann–Whitney test. ^## p < 0.05 compared with normal group and ** p < 0.01 compared with control.